#: locale=en ## Tour ### Cookies Policy CookiesPolicy_D36194F2_C212_561C_41E0_3C003D0BB844.url = cfd-productions.co.za/cookies/ ### Description tour.description = Come and have a look at the Physiology facilities on the NWU's Potchefstroom campus. ### Title tour.name = NWU - Physiology ## Skin ### Button Button_0399826A_2D79_4594_41BA_934A50D0E6B4.label = SWIMMING POOL > Button_0A054365_2D09_CB9F_4145_8C365B373D19.label = PRACTICAL LAB (2) Button_0AEB5577_2D08_CE7B_41B6_192923248F4E.label = PRACTICAL LAB (G) Button_0B73474A_2D18_CB95_41B5_180037BA80BC.label = COMPUTER LAB (2) Button_0B73474A_2D18_CB95_41B5_180037BA80BC.pressedLabel = Inserdt Text Button_1203FDB8_3106_001C_41B6_C9BE8EDD0DA9.label = Garden Button_13D4FC1E_310A_0017_41BA_DDA6D071C1BA.label = Lorem Ipsum Button_159E8DDD_31FA_0014_41C5_F18F441AF371.label = Lorem Ipsum Button_159E9DDC_31FA_0015_41B6_CB1D433C7673.label = Lorem Ipsum Button_159EBDDD_31FA_0014_41C8_935504B30727.label = Lorem Ipsum 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Company Name
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Virtual Tours by CFD Productions
cfd-productions.co.za
## Media ### Title album_9205FD97_872C_D7AA_4196_A0DFCCC11DB2.label = Photo Album station 3 album_9205FD97_872C_D7AA_4196_A0DFCCC11DB2_0.label = station 3 album_92A3B256_872D_ACAD_41C8_DCB5F7A0CEA7.label = Photo Album Station 1 album_92A3B256_872D_ACAD_41C8_DCB5F7A0CEA7_0.label = Station 1 panorama_54BC2F0A_5F7F_BF00_41CD_4BAB93E87518.label = PRACTICAL LAB (G) panorama_54C4679F_5F7C_CF01_41D2_DB649E9607AA.label = OUTSIDE panorama_54EFCE82_5F7D_4100_41C0_60D42F9E2944.label = Pano_Hallway_04 panorama_54FB3882_5F7C_C100_41B8_75CDF156C950.label = PRACTICAL LAB (2) panorama_558F8990_5F7D_C31F_41CA_C91770075E19.label = COMPUTER LAB (2) panorama_558FF42E_5F7D_C100_41D5_A5397BD9161B.label = Pano_Computerlab_01 panorama_55962DB3_5F7C_C301_41D7_4610A699D69E.label = Pano_211Praclab_02 panorama_5597C850_5F7D_411F_41D2_8F8CCE397231.label = Pano_211Praclab_03 panorama_5597F325_5F7C_C701_41AD_0C9882558768.label = Pano_211Praclab_01 panorama_559A79A2_5F7F_4303_41D6_CCCFB0C4894B.label = Pano_G14Praclab_01 panorama_559C2968_5F7F_430F_41D1_9F8D9D9F6A8B.label = Pano_G14Praclab_04 panorama_559CC43A_5F7F_4103_41D0_6C2A3FE2BC11.label = Pano_G14Praclab_02 panorama_559CCED1_5F7F_4101_41B5_85E38219D845.label = Pano_G14Praclab_03 panorama_559CE3F1_5F7F_4700_41D2_F1B96717C01B.label = Pano_G14Praclab_05 panorama_55B612E7_5F7F_4101_41BE_F3041C0AF4B7.label = Pano_Hallway_01 panorama_55B8246E_5F7F_4103_41BB_95326397F9FD.label = LECTURE HALL panorama_55B82987_5F7F_4301_41B1_E6F0BFA36523.label = Pano_Hallway_03 panorama_55BBA9E8_5F7F_4300_41C8_0EADD2F0BA27.label = Pano_Hallway_02 panorama_55BBFF06_5F7F_5F00_41C2_CAEAC1EBF159.label = Pano_Lecturehall_01 photo_94CE3F83_87EC_53AB_41AB_2CD50227DF74.label = _B2_0905 use photo_94DC06E9_87FC_D567_41D3_E9626C4763E9.label = _B2_0845 photo_960C5E0E_8714_74BD_41DE_25C7061A2BCD.label = Station 1 photo_96CBEE5A_8715_D4A5_41C5_56C7E5DA1EA2.label = _B2_0856 photo_974C2718_87FC_54A5_41BA_8FEE0E6B73DF.label = _B2_0847 photo_978DCB76_872C_B36D_41DF_B58DCBD0ED6E.label = station 2.2 photo_97AFF74F_87F4_F4BB_41DC_F623589002AC.label = _B2_0908 photo_97B311CC_872C_AFBD_41DD_E2968388AC83.label = station 4 photo_97CB0A2B_872F_FCFB_41A9_F87B4CDE6B29.label = station 3 ## Popup ### Body htmlText_600BAE09_6ECA_F04B_41B4_095761084355.html =
1. Prepare 8 test tubes with the following and label them:
• Six test tubes with 5 ml x% erythrocyte solution
(each will get a different saponin concentration: 0, 20, 40, 60, 80, 100 μg/ml)
• One test tube with 5 ml distilled water
• One test tube with 5 ml 0.9% NaCl solution (blank).
2. Now label 8 cuvettes to correspond with each test tube.
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1. Tilt test tubes carefully to mix solutions.
2. Wait 20 minutes.
3. Centrifuge the test tubes (seven in total, except the blank) for five minutes at 2000 rpm.
htmlText_88962FBD_8717_B3DE_41DD_E3DCC7576D0B.html =
1. Drip a droplet immersion oil on the feather-like side of the blood smear.
2. Set up the microscope by choosing the oil-immersion objective, securing the slide in place, and slowly elevating the table until the lens touches the oil.
3. Start at one corner and systematically scan the slide until you have counted 50 leukocytes in total.
4. Keep count of how many white blood cells (neutrophils, eosinophils, basophils, lymphocytes, and monocytes) you count - ask your friend to write it down.
5. Calculate the percentage of each cell type counted.
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1. Prick your finger and apply a small drop of blood on one end of a clean glass slide.
2. Lower another slide at an angle of 30° to the first slide, lightly touching in front of the blood droplet.
3. Gently pull the angled glass slide backwards into the drop, allowing the blood to spread out along the edge of the angled slide.
4. Quickly but smoothly push the angled slide across the bottom slide to get a thin, feathered layer of blood at the end.
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1. Add 50 μl of the erythrocyte solution to the 0 g/ml test tube (negative control) and note the time.
2. Add 50 μl of the different concentrations saponin to each of the other 5 corresponding test tubes and note the time.
3. Add 50 μl of the erythrocyte pellet to the test tube filled with 5 ml distilled water and note the time (positive control).
htmlText_89EB1AFE_8714_7D5D_41D8_A2A168E726E9.html =
1. Remove approximately 3 ml of the fluid (supernatant) from each of the test tubes and place in cuvettes.
2. Place each cuvette in the spectrophotometer then measure and note the absorbance of light at a wavelength of 540 nm for the blank and each of the other cuvettes.
3. Do calculations:
% Haemolysis = absorbance of cuvette / absorbance of distilled water x 100
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1. Flood the surface of the slide with staining solution while tilting the slide back and forth for 1-3 minutes. Be careful not to wash the blood smear off.
2. Drip distilled water on top of the stain. Be careful not to wash the stain off.
3. You should now see a metallic sheen on the surface of the stain. Leave the stain on the slide for 5 minutes.
4. Wash the diluted stain off the slide with distilled water.
5. Allow the slide to air-dry completely at an angle.
htmlText_9778FC62_87EF_F56A_41A8_924338F8A757.html =
Examine your registration details on the computer:
1. Count the amount of breath cycles per minute.
2. Use the waveform cursor and marker in the program to perform measurements as indicated by the program. The computer program will do calculations and give an output.
3. Use results in output to determine if lung volumes and capacities are normal for your sex, age and height.
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A working partner will add details on the computer and tell you when to perform each activity in the following sequence:
1. Put on the nose clip and breathe through the spirometer flow head without looking at computer. Breathe normally until you are accustomed to the apparatus.
2. Do a full expiration and then breathe normally for 1min. Then rest.
3. Try to breathe normally for 2min.
4. Inhale as deeply as possible then exhale as deeply as possible.
5. Try to breathe normally for 1min. Then rest.
6. Breathe normally for 10-20sec, then inhale and exhale as fully, forcefully, and as long as possible.
7. Breathe normally for 1min. Then stop.
### Title window_9618E258_87F4_ACA5_41CC_FE3AF7D0BC08.title = Microscopy window_96F12C19_8734_54A7_41C8_BA1B36AA3312.title = Bench preparation 1 window_972EDEB3_87EC_D5EB_41D9_F87CBCFBE821.title = Making a blood smear window_9753006A_87ED_AD65_41D1_EEE90B63213E.title = Staining of the slide window_975F2537_8737_F4EB_41BA_EC4E28EE9E0C.title = Centrifugation window_9AED89B0_899A_9E1D_41D4_602F592B5AC5.title = Spectrophotometry & Calculations window_9B46A271_8996_921F_4192_8CEB8141EDBA.title = Bench preparation 2 window_CD95AF6D_C20E_7204_41CF_DDAD25DD4967.title = Spirometry registration window_D2DC7CEF_C20E_B604_4175_FAA27B18F9D6.title = Measuring lung volumes and capacities ## Action ### URL LinkBehaviour_BB4CE89E_990C_DB96_4167_487649FE16C2.source = http://health-sciences.nwu.ac.za/physiology LinkBehaviour_ED0E472C_F8A3_944B_41C9_B69511A6CE9E.source = https://cfd-productions.co.za ## E-Learning ### Answer questionOption_9261F82C_872F_BCFD_41DA_ABB4FA43260F.text = Camera questionOption_92943958_872C_BCA6_41CD_7541C03CB477.text = Spectrophotometer questionOption_92DA7910_872C_7CA6_41E0_9C27BC721EA3.text = Centrifuge questionOption_92F6AA06_8714_7CAA_41DD_82034D47F40D.text = Distilled water test tube questionOption_937F586A_8714_FD65_4195_51B98401F399.text = 0 g/ml saponin test tube questionOption_93E2AFFB_872B_B35A_41DE_6BB627549A05.text = 0.9% NaCl test tube ### Score Name score1.label = Score ### Question question_9367D22A_872C_6CFA_41C1_9F4130D622D8.title = What apparatus is used to measure how much light is absorbed by each cuvette? question_93A8392F_8715_DCFB_41CC_CA798F0DF0B6.title = Which test tube was used as the blank? ### Question Screen quizQuestion_92FA30DD_87EC_AD5F_41D7_6B2491C728AD.ok = OK ### Report Screen quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.completion = Completed quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.downloadCSV = Download .csv quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.elapsedTime = Time quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.items = Items Found quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.questions = Questions quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.questionsCorrect = Correct quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.questionsIncorrect = Incorrect quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.repeat = Repeat quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.submitToLMS = Submit quizScore_92F850DD_87EC_AD5F_41D6_DE6E5CBE121A.title = - SCORE - ### Timeout Screen quizTimeout_92FB20DD_87EC_AD5F_41DF_2F96B0A1C1B3.repeat = Repeat quizTimeout_92FB20DD_87EC_AD5F_41DF_2F96B0A1C1B3.score = View Score quizTimeout_92FB20DD_87EC_AD5F_41DF_2F96B0A1C1B3.title = - TIMEOUT -